documentation for the confero command line interface (cli) (SourceForge net)
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Documentation For The Confero Command Line Interface (Cli), supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Confero: an integrated contrast data and gene set platform for computational analysis and biological interpretation of omics data"
Article Title: Confero: an integrated contrast data and gene set platform for computational analysis and biological interpretation of omics data
Journal: BMC Genomics
doi: 10.1186/1471-2164-14-514
Figure Legend Snippet: Biological knowledge building process enabled by Confero which enhances the standard omics analysis workflow. The left loop depicts the typical omics workflow from data generation, processing, and statistical analysis followed by downstream computational analysis and biological interpretation leading to new hypothesis generation. With Confero in place a second loop is added where statistical analysis results are automatically processed and new a priori biological knowledge in the form of gene set is stored. This database of knowledge grows with new experimental and external data and is leveraged by integrated tools for biological interpretation of current and future studies. The overall process drives and enhances the experimental research lifecycle.
Techniques Used:
Figure Legend Snippet: Confero platform overview. Depicts where Confero fits into a typical high-throughput transcriptomics analysis workflow. Contrast data is fed into the platform after the statistical analysis step where it is then converted to idMAPS format and loaded using Confero tools. Contrast data is automatically processed and stored and gene sets are extracted. Data can be analyzed for gene set enrichment and results can be used in other Confero tools or exported for other analyses.
Techniques Used: High Throughput Screening Assay
Figure Legend Snippet: Screenshot of Confero platform integrated in Galaxy. Confero platform is a standalone application that can be used via the command line. However, for non-programmatic users and to provide flexibility, Confero platform has been integrated into Galaxy. The window shows three main frames: 1) the first frame on the left contains all Confero tools (also see Additional file : Table S1) to import data (DATA IMPORT), manage and export data from Confero (DATA MANAGEMENT AND EXPORT), run GSEA or ORA and manage results (FUNCTIONAL ENRICHMENT ANALYSIS MODULE); 2) the second frame in the middle generally displays the web page with the menu when selecting a tool, and results once the job is done; 3) the third frame on the right contains all the history of actions/results performed during an analysis. This history is saved and the user can investigate the results of the analysis at any time. The user has also the possibility to share the history with other users that have an account in Galaxy.
Techniques Used: Functional Assay
Figure Legend Snippet: Screenshot of the Estrogen gene set entry in the Confero DB. Clicking on a gene set hyperlink (e.g. BMC_Bioconductor_Estrogen_PW_[Estro10]_UP) enables to access detailed information such as the rank of the genes extracted from the contrast, the Entrez gene ID, the gene symbol and description.
Techniques Used:
Figure Legend Snippet: Screenshot of the report following importing and processing of the Bioconductor estrogen contrast data in Confero using Galaxy. The report shows 1) whether contrast data have been correctly imported and processed (several checks during the mapping and collapsing process reported at the top of the document (“Check/Map/Collapse Contrast Dataset Report”), 2) information on the gene sets that have been automatically extracted from each contrast and stored (UP, DN, and AR gene sets) in the Confero DB (“Gene Set Report”). As example (see also Figure ), 169, 105 and 274 most significantly up- , down- and all-regulated genes (FDR<0.05) were respectively extracted as gene sets UP, DN and AR from the contrast data “Estro10” (comparison of gene expression levels of estrogen vs control samples collected at 10h).
Techniques Used: Comparison, Gene Expression, Control
Figure Legend Snippet: Screenshot of the Estrogen contrast dataset entry in the Confero DB. To access a database Estrogen Contrast data entry, the “View and Manage Data” tool is selected in the toolbox (left frame) and the appropriate contrast dataset name can be chosen in the available list. The entry contains information on the contrast dataset at the top (e.g. “BMC_Bioconductor_Estrogen_PW”), contrasts (e.g. “BMC_Bioconductor_Estrogen_PW_[Estro10]”) and associated gene sets at the bottom (e.g. “BMC_Bioconductor_Estrogen_PW_[Estro10]_UP”). Details and data files can be accessed via the hyperlinks. Data files can be saved locally upon needs.
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Figure Legend Snippet: Summary of Confero Bioconductor estrogen dataset GSEA results and leveraging of leading edge genes results from Export Leading Edge Matrix tool. Grouping gene sets per biological processes and investigating the leading edge genes associated to significantly enriched gene sets enables to rapidly interpret biological events at the molecular level and raise new hypothesis that could further be experimentally verified. Red and green colors highlight normalized enrichment scores that are significantly enriched for up- or down-regulated genes, respectively.
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